Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (135,145)

Singleplex and Multiplex Bead Based Immunoassays (6,198)

ELISA Substrates and Reagents (2,230)

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Invitrogen™ Mouse IL-5 Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Immunoassay Kit Format	Uncoated ELISA Kit
Content And Storage	Capture Antibody: Pre-titrated, purified antibody Detection Antibody: Pre-titrated, biotin-conjugated antibody Standard: Recombinant protein for generating standard curve and calibrating samples 10 x Coating Buffer: Buffer for plating the Capture Antibody 5 x ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme Enzyme: Pre-titrated Avidin-HRP Substrate: 1 x TMB Solution Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards 96-well Plate: Corning Costar 9018 (included with product Cat. No. ending in suffixes -22, -76, -86)
Target Species	Mouse
Conjugate	HRP
Gene Symbol	Il5
Total Assay Time	24 hr 30 min
Sample Volume	Lavage Fluid 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	8 pg/mL
Storage Requirements	2-8°C
Assay Range	8-500 pg/mL
Test Time	1 hr 45 min
Accession Number	P04401
Regulatory Status	RUO
Target	IL-5
Kit Contents	Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Avidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
Gene Alias	B-cell differentiation factor I; B-cell growth factor II; BCGF-II; colony-stimulating factor, eosinophil; cytotoxic T-lymphocyte inducer; EDF; Eosinophil differentiation factor; IL5; IL-5; ILN; Interleukin; Interleukin 5; Interleukin 5 (colony-stimulating factor eosinophil); Interleukin 5 (colony-stimulating factor, eosinophil); Interleukin5; interleukin-5; T-cell replacing factor; TRFB-cell differentiation factor I; B-cell growth factor II; BCGF-II; colony-stimulating factor, eosinophil; cytotoxic T-lymphocyte inducer; EDF; Eosinophil differentiation factor; IL5; IL-5; ILN; Interleukin; Interleukin 5; Interleukin 5 (colony-stimulating factor eosinophil); Interleukin 5 (colony-stimulating factor, eosinophil); Interleukin5; interleukin-5; T-cell replacing factor; TRF; CANDF6; IL-17F; ML-1; ML1
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	16191
Sample Type	Lavage Fluid,Serum,Supernatant
Label or Dye	Biotin

Invitrogen™ Human IL-8 Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Gene Symbol	GMCSF
Total Assay Time	24 hrs 30 mins
Sample Volume	Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	2 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	2 to 250 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Accession Number	P10145
Regulatory Status	RUO
Target	IL-8
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,Coating Buffer: 10X PBS ELISA Coating Buffer,Assay Diluent: 5X Concentrated,Detection Enzyme: Pre-titrated Avidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22 and -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3576
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Interleukin-8, CXCL8, NAP-I

Invitrogen™ Mouse IgM Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Total Assay Time	24 hrs 30 mins
Sample Volume	Plasma 10 μL, Serum 10 μL, Supernatant 50 μL
Assay Sensitivity	0.39 ng/mL
Storage Requirements	2°C to 8°C
Assay Range	0.39 to 25 ng/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Regulatory Status	RUO
Target	Mouse IgM
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	16019
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Immunoglobulin M

Invitrogen™ Mouse IgA Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Total Assay Time	24 hrs 30 mins
Sample Volume	Plasma 10 μL, Serum 10 μL, Supernatant 10 μL
Assay Sensitivity	0.39 ng/mL
Storage Requirements	2°C to 8°C
Assay Range	0.39 to 25 ng/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Regulatory Status	RUO
Target	Mouse IgA
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	238447
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Immunoglobulin A

Invitrogen™ Mouse IgA Uncoated ELISA Kit with Plates

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Total Assay Time	24 hrs 30 mins
Sample Volume	Plasma 10 μL, Serum 10 μL, Supernatant 10 μL
Assay Sensitivity	0.39 ng/mL
Storage Requirements	2°C to 8°C
Assay Range	0.39 to 25 ng/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Regulatory Status	RUO
Target	Mouse IgA
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	238447
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Immunoglobulin A

Invitrogen™ Human IL-8 Uncoated ELISA Kit with Plates

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Gene Symbol	GCP-1,GCP1,IL8,LECT,LUCT,LYNAP,MDNCF,MONAP,NAF,NAP-1,NAP1
Total Assay Time	24 hrs 30 mins
Sample Volume	Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	2 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	2 to 250 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Accession Number	P10145
Regulatory Status	RUO
Target	IL-8
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3576
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Interleukin-8, CXCL8, NAP-I

Invitrogen™ Human CD63 ELISA Kit

ELISA

Pricing & Availability
Specifications

Conjugate	HRP
Interassay CV	<12%
Intraassay CV	<10
Gene Symbol	CD63
Total Assay Time	4 hrs 45 mins
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	14 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	14 to 3,000 pg/mL
Test Time	1 hrs 20 mins
Accession Number	C75951; CD 63; CD63; CD63 antigen; CD63 antigen (melanoma 1 antigen); Cd63 molecule; CD63 protein; granulophysin; LAMP-3; Lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3; mast cell antigen AD1; ME491; melanoma 1 antigen; melanoma-associated antigen ME491; melanoma-associated antigen MLA1; MLA1; Ocular melanoma-associated antigen; OMA81H; tetraspanin-30; TSPAN30; Tspan-30
Regulatory Status	RUO
Target	CD63
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	967
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP

Invitrogen™ Human PTX-3 ELISA Kit

ELISA

Pricing & Availability
Specifications

Conjugate	HRP
Interassay CV	<12%
Intraassay CV	<10
Gene Symbol	PTX3
Total Assay Time	4 hrs 45 mins
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	0.08 ng/mL
Storage Requirements	2°C to 8°C
Assay Range	0.08 to 20 ng/mL
Test Time	1 hrs 20 mins
Accession Number	AI607804; long pentraxin 3; pentaxin related; pentaxin-related protein PT x 3; pentraxin 3; pentraxin 3, long; pentraxin related; pentraxin related gene; pentraxin-related protein PT x 3; Pt x 3; TNF alpha-induced protein 5; TNFAIP5; TSG14; TSG-14; Tumor necrosis factor alpha-induced protein 5; Tumor necrosis factor-inducible gene 14 protein; tumor necrosis factor-inducible protein TSG-14
Regulatory Status	RUO
Target	PTX3
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	5806
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP

Invitrogen™ Human FOLR1 ELISA Kit

ELISA

Pricing & Availability
Specifications

Conjugate	HRP
Interassay CV	<12%
Intraassay CV	<10
Gene Symbol	FOLR1
Total Assay Time	4 hrs 45 mins
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	0.1 ng/mL
Storage Requirements	2°C to 8°C
Assay Range	0.102 to 25 ng/mL
Test Time	1 hrs 20 mins
Accession Number	Adult folate-binding protein; FBP; Fbp1; folate binding protein; folate binding protein 1; folate receptor 1; folate receptor 1 (adult); folate receptor alpha; Folate receptor, adult; folate-binding protein 1; Folbp1; Folbp-1; FOLR; FOLR1; FR-alpha; KB cells FBP; MOv18; ovarian tumor-associated antigen MOv18
Regulatory Status	RUO
Target	Folate Receptor alpha
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	2348
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP

Invitrogen™ Human NSE ELISA Kit

ELISA

Pricing & Availability
Specifications

For Use With (Equipment)	Colorimetric Microplate Reader
Gene ID (Entrez)	2026
Interassay CV	<10%
Intraassay CV	<10
Assay Sensitivity	1.4 ng/mL
Assay Range	2.34 to 150 ng/mL

Invitrogen™ Mouse Melatonin ELISA Kit

ELISA

Pricing & Availability
Specifications

For Use With (Equipment)	Colorimetric Microplate Reader
Interassay CV	<10%
Intraassay CV	<10
Assay Sensitivity	4.69 pg/mL
Assay Range	7.81 to 500 pg/mL

R&D Systems™ Human Total Adiponectin/Acrp30 Quantikine ELISA

Human Total Adiponectin/Acrp30 Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

Pricing & Availability
Specifications

Specificity	Natural and Recombinant Human total Adiponectin (low, middle, and high molecular weight)
Target Species	Human
Conjugate	HRP
Product Type	Solid Phase Sandwich ELISA
Gene ID (Entrez)	9370
Sample Type	Cell Culture Supernatant,Plasma (EDTA),Plasma (Heparin),Serum
Sample Volume	Cell Culture Supernates (50uL),Serum (10uL),EDTA Plasma (10uL),Heparin Plasma (10uL)
Synonym	ACDC, Acrp30, ACRP30ADPN, adipocyte, C1Q and collagen domain containing, adiponectin, adiponectin, C1Q and collagen domain containing, AdipoQ, ADIPQTL1, apM-1, ApM1, APM1APM-1, C1q and collagen domain-containing protein, GBP28, GBP28apM1, Gelatin-binding protein
Assay Sensitivity	0.891ng/mL
Storage Requirements	Store the unopened product at 2° to 8°C. Do not use past expiration date.
Assay Range	3.90 - 250ng/mL
For Use With (Application)	ELISA
Cross Reactivity	<50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules.

R&D Systems™ Human GDF-15 Quantikine ELISA Kit

Human GDF-15 Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.